{"id":12142,"date":"2024-07-19T19:37:55","date_gmt":"2024-07-19T19:37:55","guid":{"rendered":"https:\/\/nemistech.keepnetics.com\/2024\/07\/19\/rapid-testing-methods-for-e-coli-monitoring-in-food-production\/"},"modified":"2025-04-10T10:21:20","modified_gmt":"2025-04-10T10:21:20","slug":"rapid-testing-methods-for-e-coli-monitoring-in-food-production","status":"publish","type":"post","link":"https:\/\/nemistech.keepnetics.com\/en-us\/blog\/rapid-testing-methods-for-e-coli-monitoring-in-food-production\/","title":{"rendered":"Rapid testing methods for <em>E. coli<\/em> monitoring in food production"},"content":{"rendered":"<p><span data-contrast=\"none\">Ensuring food safety is a critical concern in the food production industry. Contaminated food products can pose health risks to consumers, resulting in product recalls and damage to brand reputation. In this article, we will provide an overview of <\/span><i><span data-contrast=\"none\">E. coli <\/span><\/i><span data-contrast=\"none\">testing methods <\/span><span data-contrast=\"none\">and <em>E. coli&#8217;s<\/em> risks in food production and highlight the importance of rapid testing methods for effective monitoring. <\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><!--more--><\/p>\n<h2 aria-level=\"2\"><span data-contrast=\"none\">Overview of <\/span><i><span data-contrast=\"none\">E. coli <\/span><\/i><span data-contrast=\"none\">and Its Risks in Food Production<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;201341983&quot;:0,&quot;335559738&quot;:40,&quot;335559739&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/h2>\n<p><span data-contrast=\"none\">The origin of <\/span><i><span data-contrast=\"none\">E. coli <\/span><\/i><span data-contrast=\"none\">in nearly all food and water contamination cases can be attributed to exposure to fecal contamination, which can occur through multiple sources, such as farms where cattle carry <\/span><i><span data-contrast=\"none\">E. coli <\/span><\/i><span data-contrast=\"none\">in their gut. In addition, wildlife, such as deer, birds, and pests in agricultural areas, can extract feces containing <\/span><i><span data-contrast=\"none\">E. coli <\/span><\/i><span data-contrast=\"none\">on plants, leading to potential contamination.\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">Raw milk can be contaminated with <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> from cattle feces during milking. Furthermore, during cattle slaughter, <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> from wastes can occasionally contaminate beef, typically on the surface of the meat.\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">Cooking can often eliminate the bacteria in many cuts of beef, such as steak. Ground meat, however, poses a higher risk as the grinding process can mix and distribute the contaminated meat throughout the product, making thorough cooking essential.\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">Poor personal hygiene can also be a potential source of fecal contamination. For instance, failure to wash hands properly after using the toilet can transfer harmful <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> from infected food handlers to food products, leading to potential contamination.\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">Traditional methods for <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> testing in food production often involve time-consuming and laboratory-intensive processes that must\u00a0be carried out in a specially equipped microbiology laboratory. Often incubation of samples for 24-48 hours is required before results can be obtained.\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<h2 aria-level=\"2\"><span data-contrast=\"none\">Rapid, lab-free detection of <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> with a novel NEMIS N-Light<\/span><sup><span data-contrast=\"none\">TM<\/span><\/sup><span data-contrast=\"none\"> test\u00a0<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;201341983&quot;:0,&quot;335559738&quot;:40,&quot;335559739&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/h2>\n<p><span data-contrast=\"none\">The novel <a href=\"https:\/\/www.nemistech.com\/technology\/\">N-Light\u2122<\/a> <a href=\"https:\/\/www.nemistech.com\/products\/e-coli-rapid-test\/\"><em>E. coli<\/em> test<\/a><\/span><span data-contrast=\"none\">\u00a0is a rapid qualitative method for detecting <\/span><i><span data-contrast=\"none\">Escherichia coli<\/span><\/i><span data-contrast=\"none\"> as a hygiene indicator in food processing. This test can monitor and control factors such as compliance with hand hygiene rules, hygiene status of incoming materials (e.g., potential contamination with manure residues, etc.), and effectiveness of sterilization steps in food processing. It is suitable for use in food processing areas and equipment as part of an environmental monitoring program and for testing process water and other liquid samples.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\"> The E.coli rapid test was designed by <a href=\"https:\/\/www.nemistech.com\/\">NEMIS<\/a> to be user-friendly and versatile, serving as a hygiene indicator for various settings, including factory surfaces, tools, production machines, and employee hand hygiene. Unlike agar plates and complex sample preparation, this test offers a convenient solution, delivering reliable results within 16 hours.<\/span><\/p>\n<p><img decoding=\"async\" style=\"height: auto; max-width: 100%; width: 1374px;\" src=\"https:\/\/25392440.fs1.hubspotusercontent-eu1.net\/hubfs\/25392440\/Rapid%20testing%20methods%20for%20e.coli%20monitoring%20in%20foo.png\" alt=\"Rapid testing methods for e.coli monitoring in foo\" width=\"1374\" height=\"377\" \/><\/p>\n<p><span data-contrast=\"none\">The advantages of the N-Light\u2122 E. coli test are:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Lab-free testing<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"none\">Take action after only 16 hours.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"3\" data-aria-level=\"1\"><span data-contrast=\"none\">Anyone can do it<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"4\" data-aria-level=\"1\"><span data-contrast=\"none\">Closed system from sampling to detection<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"4\" data-aria-level=\"1\"><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">Digital results in 4 simple steps\u00a0\u00a0<\/span><\/li>\n<\/ul>\n<h2 aria-level=\"2\"><span data-contrast=\"none\">Other <em>E. coli<\/em> testing methods<\/span><span data-contrast=\"none\">\u00a0used in the food industry:\u00a0<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;201341983&quot;:0,&quot;335559738&quot;:40,&quot;335559739&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/h2>\n<h3 aria-level=\"3\"><span data-contrast=\"none\">Polymerase Chain Reaction (PCR)<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;201341983&quot;:0,&quot;335559738&quot;:40,&quot;335559739&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/h3>\n<p><span data-contrast=\"none\">PCR is a molecular-based technique that amplifies the DNA of <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> in food samples, allowing for highly sensitive and specific detection of this bacteria. PCR needs enrichment of bacteria before analysis, typically requiring an incubation step of 16 to 24 h in the laboratory. DNA extraction involves several working steps to ensure reproducible and reliable results.\u00a0 PCR requires personnel specifically trained in molecular biology methods and a set of instruments (incubator, micropipettes, laminar flow bench, centrifuge, thermocycler, and DNA electrophoresis equipment or qPCR device).<\/span><\/p>\n<p><span data-contrast=\"none\"><img decoding=\"async\" style=\"height: auto; max-width: 100%; width: 899px;\" src=\"https:\/\/25392440.fs1.hubspotusercontent-eu1.net\/hubfs\/25392440\/Rapid%20testing%20methods%20for%20e.coli%20monitoring%20in%20foo-1.png\" alt=\"Rapid testing methods for e.coli monitoring in foo-1\" width=\"899\" height=\"654\" \/><\/span><\/p>\n<h3 aria-level=\"3\"><span data-contrast=\"none\">Solid Phase Cytometry<\/span><\/h3>\n<p aria-level=\"3\"><i style=\"background-color: transparent;\"><span data-contrast=\"none\">E.coli<\/span><\/i><span data-contrast=\"none\"> can be detected using solid-phase cytometry (SPC) in combination with fluorescent viability staining. In this method, water samples containing <\/span><i style=\"background-color: transparent;\"><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> are filtered through a polyester membrane with a pore size of 0.4 \u00b5m under vacuum to capture the bacteria. The retained <\/span><i style=\"background-color: transparent;\"><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> on the filter is then treated with reagents to induce the enzyme \u03b2-D-glucuronidase and incubated at 37\u2070C for 3 hours. The induced cells are then labelled, with only viable cells able to mark the enzyme, causing the bacteria to cleave the non-fluorescent substrate and leaving only the fluorescent end inside the viable cells.<\/span><\/p>\n<p><span data-contrast=\"none\">The advantages of Solid Phase Cytometry for <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> detection are:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Ability to detect viable organisms, providing a more accurate assessment of bacterial presence.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"none\">Relatively low cost per test, making it cost-effective for routine testing.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"3\" data-aria-level=\"1\"><span data-contrast=\"none\">Rapid and visual detection of contaminated water using the ScanRDI\u00ae device.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><span data-contrast=\"none\">The limitations of Solid Phase Cytometry for <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> detection are:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Limited to detecting viable organisms only and may not detect non-viable or dormant cells.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"none\">Reliance on fluorescent signal, which may have limitations in sensitivity and specificity.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"3\" data-aria-level=\"1\"><span data-contrast=\"none\">Involves a multi-step process, requiring careful handling and coordination of different steps.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"4\" data-aria-level=\"1\"><span data-contrast=\"none\">Additional steps, such as biochemical tests or mass spectrometry, may be needed to confirm <\/span><i><span data-contrast=\"none\">E.coli<\/span><\/i><span data-contrast=\"none\"> presence.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<h3 aria-level=\"3\"><span data-contrast=\"none\">Viable Cell Counting<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;201341983&quot;:0,&quot;335559738&quot;:40,&quot;335559739&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/h3>\n<p><span data-contrast=\"none\">There are various methods to measure bacterial concentrations, and a straightforward approach is <\/span><strong><span data-contrast=\"none\">viable cell counting<\/span><\/strong><span data-contrast=\"none\"> on agar plates. In this method, the bacterial sample is serially diluted (1:10, 1:100, 1:1000, etc.) in sterile water or Phosphate Buffer Saline (PBS) and plated on semi-selective media for isolation of <\/span><i><span data-contrast=\"none\">Escherichia coli<\/span><\/i><span data-contrast=\"none\">, e.g. chromogenic agar plates containing synthetic enzyme substrate X-Glucuronide. The plates are incubated overnight at 37\u00b0C. Further biochemical tests or MALDI-TOF may be performed on pure isolated colonies to confirm the presence of <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> and differentiate it from other organisms.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The advantages of viable cell counting on agar plates are:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Ability to detect viable organisms, providing a direct measure of culturable bacterial concentration.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"none\">Relatively low cost per test, making it cost-effective for routine testing.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><span data-contrast=\"none\">The limitations of viable cell counting on agar plates are:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Limited to detecting viable organisms that can grow on the chosen media may not capture non-culturable or slow-growing bacteria.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"none\">Reliance on colony formation, which may have limitations in sensitivity and specificity.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"3\" data-aria-level=\"1\"><span data-contrast=\"none\">Involves a multi-step process, requiring dilution, plating, and incubation steps.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"2\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559684&quot;:-2,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"4\" data-aria-level=\"1\"><span data-contrast=\"none\">Additional steps, such as biochemical tests or mass spectrometry, may be needed to confirm <\/span><i><span data-contrast=\"none\">E. coli<\/span><\/i><span data-contrast=\"none\"> presence.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<h5><span data-contrast=\"auto\">References:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/h5>\n<ol>\n<li><a href=\"https:\/\/starfishmedical.com\/blog\/best-method-detect-e-coli\/\"><span data-contrast=\"none\">https:\/\/starfishmedical.com\/blog\/best-method-detect-e-coli\/<\/span><\/a><span data-contrast=\"auto\">\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><a href=\"http:\/\/ib.bioninja.com.au\/standard-level\/topic-3-genetics\/35-genetic-modification-and\/pcr.html\"><span data-contrast=\"none\">http:\/\/ib.bioninja.com.au\/standard-level\/topic-3-genetics\/35-genetic-modification-and\/pcr.html<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><a href=\"https:\/\/www.researchgate.net\/publication\/42388104_Detection_of_Escherichia_coli_O157H7_Using_Gold_Nanoparticle_Labeling_and_Inductively_Coupled_Plasma_Mass_Spectrometry\"><span data-contrast=\"none\">https:\/\/www.researchgate.net\/publication\/42388104_Detection_of_Escherichia_coli_O157H7<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><a href=\"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-009-1879-x.pdf\"><span data-contrast=\"none\">https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-009-1879-x.pdf<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11777581\"><span data-contrast=\"none\">https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11777581<\/span><\/a><span data-contrast=\"none\">\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><a href=\"https:\/\/courses.lumenlearning.com\/boundless-microbiology\/chapter\/counting-bacteria\/\"><span data-contrast=\"none\">https:\/\/courses.lumenlearning.com\/boundless-microbiology\/chapter\/counting-bacteria\/<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ensuring food safety is a critical concern in the food production industry. Contaminated food produc&#8230;<\/p>\n","protected":false},"author":1,"featured_media":11943,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[150],"tags":[136],"class_list":["post-12142","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","tag-environmental-monitoring-en-us"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Rapid E. 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